更新時間:2016-05-10
我司BRL大鼠肝細(xì)胞,產(chǎn)地美國ATCC細(xì)胞庫,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測,確保細(xì)胞無污染,符合檢測合格標(biāo)準(zhǔn)。訂購7~15 天內(nèi)即可發(fā)貨,采用快遞郵寄,確保細(xì)胞Z短時間內(nèi)送到客戶手中。
免費(fèi)咨詢:021-54720761
BRL大鼠肝細(xì)胞培養(yǎng)注意事項:
1、實驗進(jìn)行前,無菌室及無菌操作臺(laminarflow)以紫外燈照射30-60分鐘滅菌,以70%ethanol擦拭無菌操作抬面,并開啟無菌操作臺風(fēng)扇運(yùn)轉(zhuǎn)10分鐘后,才開始實驗操作。
2、BRL大鼠肝細(xì)胞操作工作區(qū)域應(yīng)保持清潔及寬敞,必要物品,例如試管架、吸管吸取器或吸管盒等可以暫時放置,其它實驗用品用完即應(yīng)移出,以利于氣流之流通。實驗用品以70%ethanol擦拭后才帶入無菌操作臺內(nèi)。
3、小心取用無菌之實驗物品,避免造成污染。勿碰觸吸管尖頭部或是容器瓶口,亦不要在打開之容器正上方操作實驗。容器打開后,以手夾住瓶蓋并握住瓶身,傾斜約45°角取用,盡量勿將瓶蓋蓋口朝上放置桌面。
3、工作人員應(yīng)注意自身之安全,須穿戴實驗衣及手套后才進(jìn)行實驗。
目錄號 | CC-Y3004 |
細(xì)胞英文(簡稱) | BRL |
細(xì)胞名稱 | |
背景資料 | 收到細(xì)胞,請查看瓶子是否有破裂,培養(yǎng)基是否漏出,是否渾濁,如有請盡快和我們。如包裝完好,取出25cm2培養(yǎng)瓶,75%酒精消毒,拆下封口膜,在顯微鏡下觀察細(xì)胞形態(tài)是否正常,細(xì)胞的貼壁情況以及細(xì)胞密度,然后放入37℃,5%CO2細(xì)胞培養(yǎng)箱中靜置2-3小時,以穩(wěn)定細(xì)胞狀態(tài)。 |
細(xì)胞來源 | ATCC |
代次 | P3 |
規(guī)格 | T25 |
細(xì)胞數(shù) | 50-100萬 |
價格 | 電議 |
生物安全級別 | 2 |
組織來源 | 肝 |
細(xì)胞形態(tài) | 貼壁;上皮細(xì)胞樣 |
細(xì)胞活力 | 95%(Viability by Trypan Blue Exclusion) |
細(xì)胞檢測 | 細(xì)胞不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌 |
培養(yǎng)條件 | RPMI-1640 +10% FBS;37℃,5% CO2 |
傳代方法 | 建議1:2-1:3 兩天換液一次 |
凍存條件 | *培養(yǎng)基50%+40%FBS+10%DMSO |
提供的細(xì)胞類型有細(xì)胞系,每個細(xì)胞發(fā)貨前都必須經(jīng)過實驗室QC檢測,確保每個批次的質(zhì)量。是上海生物行業(yè)*的代理供應(yīng)商,提供報價、咨詢及技術(shù)服務(wù),選購。
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